aggregation of vesicles

We employed dynamic and static light scattering to study the fusion of phosphatidylcholinevesiclesin the presence of model fusion peptide fragments from the hemagglutinin HA2 protein. The fusion-inducedaggregationunder the present experimental setup exhibited strong pH dependence, similar to the parental viral protein.

Jan 01, 1983· Membrane fusion andaggregationof phospholipidvesiclesare reviewed and discussed. The fusion process is viewed as consisting of several stages:aggregationand close apposition of the particles, destabilization, and finally, merging of the bilayers.

Jun 22, 2005·Vesiclesfrom SH-SY5Y cells also showed rapid α-synaggregationcompared with the cytosol (data not shown). These vesicular aggregates were formed from the intravesicular α-syn rather than the trace amounts of cytosolic contaminants orvesicle-surface-bound α-syn, because the release of lumenal α-syn completely abolished de novoaggregation...

Thevesicle aggregationreaction can be reconstituted: liposomes of phospholipid composition approximating that found on the cytoplasmic surfaces of the plasma membrane and of coatedvesicles(70% L-alpha-phosphatidylethanolamine (type I-A), 15% L-alpha-phosphatidyl-L-serine, and 15% L-alpha-phosphatidylinositol) aggregated after addition of AP ...

Nov 01, 1996· Notably, possibleaggregation of vesiclesin these compartmentalized systems was not investigated. The current study is devoted tovesicle aggregationin- duced by dextran and PEG over a wide range ofmolecular weights andconcentrations ofthepolymers as afunctionof the temperature and of the size and concentration of thevesicles.

Lipidvesiclesare widely used as model systems to study biological membranes. The self-assembly of suchvesiclesintovesiclepairs provides further opportunity to study interactions between membranes. However, formationof vesiclepairs, while subsequently keeping their colloidal stability intact, is chall

The surface modificationof vesicleswith glycolipids (O 1,O 5-bis(octadecyl) N-maltooligonoyl-l-glutamate) accelerates theaggregation of vesiclesinduced by dextran; therefore, M c significantly decreased due to the surface modification.

Feb 02, 2015· α-Synuclein (α-syn) is a 140-residue intrinsically disordered protein that is involved in neuronal and synapticvesicleplasticity, but itsaggregationto form amyloid fibrils is the hallmark of ...

Nov 08, 2016· However, extracellularvesiclessuffer fromaggregationand flocculation when stored in physiological saline solutions and degrade during freezing/thawing. It …

Aggregationof E. colivesicleswas not observed when 2 mM MgCl 2 was introduced to thevesicledispersion (White et al., 2000), as the authors used a phosphate buffer system in contrast to Na-HEPES buffer used for dilution in this study. In addition, the originalvesicledispersion contained much biggervesicleswith hydrodynamic diameters ...

As a function of size,aggregationdecreased slightly from small to 700 A diametervesiclesand increased considerably for 950 A diametervesicles. A model of the interaction of smallvesiclesbelow the gel-liquid-crystalline phase transition is presented in whichaggregationprecedes fusion and collision between aggregates triggers fusion.

Aggregationof thesevesicleswas induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze−fracture ...

The extent ofaggregationwas similar at 37 and 4 degrees C.Aggregationof large unilamellarvesiclesrequired a lower polymer concentration, probably becauseaggregationoccurs in a secondary minimum (without surface contact).

Thevesicle aggregationreaction can be reconstituted: liposomes of phospholipid composition approximating that found on the cytoplasmic surfaces of the plasma membrane and of coatedvesicles(70% L-alpha-phosphatidylethanolamine (type I-A), 15% L-alpha-phosphatidyl-L-serine, and 15% L-alpha-phosphatidylinositol) aggregated after addition of AP ...

Membrane fusion andaggregationof phospholipidvesiclesare reviewed and discussed. The fusion process is viewed as consisting of several stages:aggregationand close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11 >) and the rate constant for fusion of the dimers (f ...

Finally, Bcl‐x L, an apoptotic antagonist in programmed cell death, can prevent theaggregationof LUVs induced by tBID, but not the release of entrapped trypsin. It is postulated that two separate domains of tBID are responsible for inducing leakage andaggregationof phospholipidvesicles.

The surface modificationof vesicleswith glycolipids (O 1,O 5-bis(octadecyl) N-maltooligonoyl-l-glutamate) accelerates theaggregation of vesiclesinduced by dextran; therefore, M c significantly decreased due to the surface modification.

May 01, 2002· Theaggregationkinetics of an electrostatically stabilized dipalmitoyl phosphatidylcholinevesiclesystem. Journal of Colloid and Interface Science 1989, 132 (1) , 125-138. DOI: 10.1016/0021-9797(89)90223-3. Jin Keun Lee, Stephen E. Schullery. Effect of membrane additives onvesiclefusion.

Aggregationof thesevesicleswas induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze-fracture transmission electron microscopy shows that almost noaggregationoccurred in solutions with no added electrolyte, in which the Debye length was much ...