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aggregation of vesicles

Dec 23, 2015· Keywords: extracellularvesicles, blood plasma, cryo-electron microscopy, immuno-gold electron microscopy, flow cytometry,vesicle aggregation, centrifugation Blood and other body fluids contain membranevesiclesthat are released by cells upon activation or apoptosis ( 1 – 3 ).

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Fusion induced aggregation of model vesicles studied by
Fusion induced aggregation of model vesicles studied by

We employed dynamic and static light scattering to study the fusion of phosphatidylcholinevesiclesin the presence of model fusion peptide fragments from the hemagglutinin HA2 protein. The fusion-inducedaggregationunder the present experimental setup exhibited strong pH dependence, similar to the parental viral protein.

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Aggregation and fusion of phospholipid vesicles
Aggregation and fusion of phospholipid vesicles

Jan 01, 1983· Membrane fusion andaggregationof phospholipidvesiclesare reviewed and discussed. The fusion process is viewed as consisting of several stages:aggregationand close apposition of the particles, destabilization, and finally, merging of the bilayers.

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Intravesicular Localization and Exocytosis of α Synuclein
Intravesicular Localization and Exocytosis of α Synuclein

Jun 22, 2005·Vesiclesfrom SH-SY5Y cells also showed rapid α-synaggregationcompared with the cytosol (data not shown). These vesicular aggregates were formed from the intravesicular α-syn rather than the trace amounts of cytosolic contaminants orvesicle-surface-bound α-syn, because the release of lumenal α-syn completely abolished de novoaggregation...

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Clathrin assembly protein AP 2 inducesaggregationof
Clathrin assembly protein AP 2 inducesaggregationof

Thevesicle aggregationreaction can be reconstituted: liposomes of phospholipid composition approximating that found on the cytoplasmic surfaces of the plasma membrane and of coatedvesicles(70% L-alpha-phosphatidylethanolamine (type I-A), 15% L-alpha-phosphatidyl-L-serine, and 15% L-alpha-phosphatidylinositol) aggregated after addition of AP ...

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Aggregation of phospholipid vesicles by water soluble
Aggregation of phospholipid vesicles by water soluble

Nov 01, 1996· Notably, possibleaggregation of vesiclesin these compartmentalized systems was not investigated. The current study is devoted tovesicle aggregationin- duced by dextran and PEG over a wide range ofmolecular weights andconcentrations ofthepolymers as afunctionof the temperature and of the size and concentration of thevesicles.

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Self limiting aggregation of phospholipid vesicles   Soft
Self limiting aggregation of phospholipid vesicles Soft

Lipidvesiclesare widely used as model systems to study biological membranes. The self-assembly of suchvesiclesintovesiclepairs provides further opportunity to study interactions between membranes. However, formationof vesiclepairs, while subsequently keeping their colloidal stability intact, is chall

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Critical Molecular Weight Effects in theAggregationof
Critical Molecular Weight Effects in theAggregationof

The surface modificationof vesicleswith glycolipids (O 1,O 5-bis(octadecyl) N-maltooligonoyl-l-glutamate) accelerates theaggregation of vesiclesinduced by dextran; therefore, M c significantly decreased due to the surface modification.

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Lipidvesiclestrigger α synucleinaggregationby
Lipidvesiclestrigger α synucleinaggregationby

Feb 02, 2015· α-Synuclein (α-syn) is a 140-residue intrinsically disordered protein that is involved in neuronal and synapticvesicleplasticity, but itsaggregationto form amyloid fibrils is the hallmark of ...

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Trehalose preventsaggregationof exosomes and cryodamage
Trehalose preventsaggregationof exosomes and cryodamage

Nov 08, 2016· However, extracellularvesiclessuffer fromaggregationand flocculation when stored in physiological saline solutions and degrade during freezing/thawing. It …

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Frontiers | Calcium Dependent ReversibleAggregationof
Frontiers | Calcium Dependent ReversibleAggregationof

Aggregationof E. colivesicleswas not observed when 2 mM MgCl 2 was introduced to thevesicledispersion (White et al., 2000), as the authors used a phosphate buffer system in contrast to Na-HEPES buffer used for dilution in this study. In addition, the originalvesicledispersion contained much biggervesicleswith hydrodynamic diameters ...

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Aggregationof dipalmitoylphosphatidylcholinevesicles
Aggregationof dipalmitoylphosphatidylcholinevesicles

As a function of size,aggregationdecreased slightly from small to 700 A diametervesiclesand increased considerably for 950 A diametervesicles. A model of the interaction of smallvesiclesbelow the gel-liquid-crystalline phase transition is presented in whichaggregationprecedes fusion and collision between aggregates triggers fusion.

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Self limiting aggregation of phospholipid vesicles
Self limiting aggregation of phospholipid vesicles

Aggregationof thesevesicleswas induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze−fracture ...

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Aggregation of phospholipid vesicles by water soluble
Aggregation of phospholipid vesicles by water soluble

The extent ofaggregationwas similar at 37 and 4 degrees C.Aggregationof large unilamellarvesiclesrequired a lower polymer concentration, probably becauseaggregationoccurs in a secondary minimum (without surface contact).

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Clathrin assembly protein AP 2 induces aggregation of
Clathrin assembly protein AP 2 induces aggregation of

Thevesicle aggregationreaction can be reconstituted: liposomes of phospholipid composition approximating that found on the cytoplasmic surfaces of the plasma membrane and of coatedvesicles(70% L-alpha-phosphatidylethanolamine (type I-A), 15% L-alpha-phosphatidyl-L-serine, and 15% L-alpha-phosphatidylinositol) aggregated after addition of AP ...

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Aggregation and fusion of phospholipid vesicles   NASA/ADS
Aggregation and fusion of phospholipid vesicles NASA/ADS

Membrane fusion andaggregationof phospholipidvesiclesare reviewed and discussed. The fusion process is viewed as consisting of several stages:aggregationand close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11 >) and the rate constant for fusion of the dimers (f ...

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Leakage andaggregationof phospholipidvesiclesinduced
Leakage andaggregationof phospholipidvesiclesinduced

Finally, Bcl‐x L, an apoptotic antagonist in programmed cell death, can prevent theaggregationof LUVs induced by tBID, but not the release of entrapped trypsin. It is postulated that two separate domains of tBID are responsible for inducing leakage andaggregationof phospholipidvesicles.

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Critical Molecular Weight Effects in theAggregationof
Critical Molecular Weight Effects in theAggregationof

The surface modificationof vesicleswith glycolipids (O 1,O 5-bis(octadecyl) N-maltooligonoyl-l-glutamate) accelerates theaggregation of vesiclesinduced by dextran; therefore, M c significantly decreased due to the surface modification.

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Aggregation of dipalmitoylphosphatidylcholine vesicles
Aggregation of dipalmitoylphosphatidylcholine vesicles

May 01, 2002· Theaggregationkinetics of an electrostatically stabilized dipalmitoyl phosphatidylcholinevesiclesystem. Journal of Colloid and Interface Science 1989, 132 (1) , 125-138. DOI: 10.1016/0021-9797(89)90223-3. Jin Keun Lee, Stephen E. Schullery. Effect of membrane additives onvesiclefusion.

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Electrostatic control of spontaneousvesicle aggregation
Electrostatic control of spontaneousvesicle aggregation

Aggregationof thesevesicleswas induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze-fracture transmission electron microscopy shows that almost noaggregationoccurred in solutions with no added electrolyte, in which the Debye length was much ...

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